Fig. 9. Mutant G1961E does not colocalize at the plasma membrane but is rescued by VX-809 (A) G1961E ABCA4-GFP (green) stably transfected cells were plated on coverslips. Cells were subsequently fixed and stained with DAPI (blue, nucleus) and antibodies against cadherin (red, plasma membrane). Representative extended focus images are shown. (B): Mutant G1961E is rescued to the plasma membrane following treatment with VX-809. G1961E ABCA4-GFP (green) stably transfected cells were plated on coverslips. Cells were subsequently fixed and stained with DAPI (blue, nucleus) and antibodies against cadherin (purple, plasma membrane). 3 separate experiments were performed. Representative extended focus images are shown. Colocalization signal is white (white arrows). (C): Pearson's coefficients were obtained for VX-809 treated and untreated cells. Coefficients were compared using the student's t-test and found to be significant. Conclusion: VX-809 restores surface expression of the mutant.